> To: immunology at net.bio.net> From: mkuiper at hgmp.mrc.ac.uk (Mr. M. Kuiper)
> Subject: antibody biotinylation
> Date: 31 Oct 1994 16:14:55 -0000
> Dear immuno-netters,
>> Can anybody out there tell me whether it is possible to
> biotinylate antibodies yourself. If anyone could suggest
> an efficient protocol I would be most grateful. I want
> to use the biotinylated antibodies in ELISAs.
>> Thanks for your help,
> *******************************************************************
> * *
> * Marcel Kuiper *
> * King's College School of Medicine & Dentistry *
> * Department of Molecular Medicine *
> * Rayne Institute, 2nd floor *
> * 123 Coldharbour Lane *
> * London SE5 9NU, UK *
> * Fax: 44-71-733 3877 *
> * Email: mkuiper at hgmp.mrc.ac.uk *
> * rchz103 at bay.cc.kcl.ac.uk *
> * *
> * 'The first myth of management is that it exists'. Murphy's Law. *
> * *
> *******************************************************************
>>Yes, its pretty easy to do. There is a protocol in Lane,
"Antibodies: A Laboratory Manual". If you don't have access to the
book, let me know and I'll emai you the protocol I use.
Frederick C. Garbrecht
fred at bmt.mcw.edu
Bone Marrow Transplant Program
Medical College of Wisconsin
phone 414 257 5053
fax 414 257 7994
