In article <34qqol$4ck at search01.news.aol.com>, biotecburd at aol.com
(BiotecBurd) writes:
Subject: Re: Biotinylation of Ab
From: biotecburd at aol.com (BiotecBurd)
Date: 9 Sep 1994 19:23:01 -0400
Message-ID: <34qqol$4ck at search01.news.aol.com>
In article <prudence.grandison-090994160516 at 202.36.157.201>,
prudence.grandison at genesis.co.nz (Prudence Grandison) writes:
>>Usually an equal molar ratio of biotin to protein works well when using
activated biotin molecules (e.g., biotin- carboxy- methyl-N-
hydroxyusuccinimide ester). If you find that you are getting high
background using biotin/streptavidin systems, dry labelling your protein
with dig-NHS. The anti-dig Fab conjugates do not stick non-specifically
and result in low background.<<
What is the [dig] in [dig-NHS]? I am always looking for ways to reduce
background. Much obliged.
Geoffrey D. Wheelock
Ithaca, NY
