IUBio

Obtaining anti-human IgE for elisa spot assay

Christopher Spry cspry at place.demon.co.uk
Mon Sep 12 09:10:46 EST 1994


A colleague is setting up an assay to count human lymphocytes secreting
IgE to a defined antigen using an Elisa spot assay. He would like to
know if there are any anti-IgE antibodies that he could purchase or
obtain to do this. The indicator system he plans to use is an alkaline
phosphatase-linked second antibody. Please email him (Dr. David Lewis)
if you can help: sgjf300 at sghms.ac.uk

Thanks for any help you can give.

Christopher Spry



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