I am currently trying to isolate macrophages from the anterior kidneys of
juvenile salmon using discontinuous Percoll gradients followed by
adherence to plastic multiwell plates. I have sucessfully recovered
phagocytes, but my problem is that I don't get enough of them and I can't
get them reliably. Sometimes i get as many as 1-2 * 10^6 cells from a
fish but more often I only recover 10^5 from a similar fish at the same
time. I would be interested in hearing from anyone who has worked with
percoll gradients or fish phagocytes who might know what factors might be
affecting my cell yield. Thanks.
Jimmy Pegg
jimmy at unixg.ubc.ca
