Since I posted a message "Abs without immunization", I received some
responses. I thank you for your attention.
The protocol consists of using monocellular eucaryotic system to select
and express antibodies with high affinity to an antigen coexpressed in vivo.
No selection with antigen bound to nitrocellulose is needed as the phage
display system.
Please do not send email to me if you are just curious about the technique.
I only discuss this with people who recognize the importance and potential
value of the system.
Shuang Liang, Ph.D
(216) 368-5183
