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In article <3668uc$g7e at info.curtin.edu.au>, COZZIE <icostant at info.curtin.edu.au> writes: You can use 0.3 M CuCl2 to visualize bands without fixing them. Refer to *Antibodies* by Harlow and Lane pg 65 for a protocol or Lee et al. Analyt. Biochem. 166: 308-312 (1987)