gws3u at AVERY.MED.VIRGINIA.EDU (Gail Sullivan) wrote:
>I need to be able to distinguish a dog eos from a PMN on the FACS. Does
>anyone know what tag to use? For human cells we use anti-CD16 to identify
>PMN. Does anyone know a source for dog anti-CD16 or anything else that
>might work?
>>Thank you,
>>Gail
>Gail,
I believe there is a company in the northwest called VMRD (or something like that)
that carries lots of animal antibodies, and I would bet they have one for K9 eos.
I can probably track down their number if you haven't already checked with them.
The other idea is to use depolarized 90 degree scatter--there is a description of the
setup in Howard Shapiro's PRACTICAL FLOW CYTOMETRY. I'll be able to tell
you more soon, cause I'm going to be trying this with cats, (as soon as I get the
filters!) Good luck,
Steve
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Steve G. Hilliard : "Life's too short
Cell Analysis Facility : to drink bad beer!"
University of Georgia :
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