I'm currently involved on a project attempting to elicit CD4 CTLs in
BALB/c specific to epitope-possessing synthetic peptides. To date we have
immunized mice by i.p. injection of peptide/CFA followed by 2 additional
injections of peptide/IFA. Injections were approx. 1 wk. apart.
Secondly, we tried immunizing by i.v. injections of peptide/PBS. Again,
we used a total of 3 injections approx. 1 wk. apart. Both protocols used
peptide at 100ug/injection. These attempts appear unsuccessful.
Would appreciate any information to head us in the right direction.
Zane Neal
Univ. Wisc.-Madison
Dept. of Animal Health and Biomedical Sciences
zcn at ahabs.wisc.edu
