Our lab has made hundreds of poly clonal antibodies in mice, mostly in
the past so dim only I remember it. The only reason to use a mouse is to
make a hybridoma after ab is porduced. Some one with a lot of proactice
can bleed a mouse every other day for 4 times rest a repeat it for
months, it is not a good way to make the antibody. At best you can get
2-300ul of blood from a mice yielding 100ul of serum/bleed. Change your
detection kits. Its cheaper in the long run. If you don't do this get a
company to make the antibody for you. It saves filling out all the
animal forms. When we did this we immuniozed froups of 10-15 mice, and
pooled serum.
To directly answer your questions 10-100ug/mouse of protein in adjuvant
(we have had pretty good luck with RIBI) three or four times two to
three weeks apart its usually pretty effective for inducing antibody.
BTWcost for a BALB/c from Jackson is $18.00/mouse. Can this really be
cost effective.
On Tue, 22 Aug 1995, Vidyani Manatunja wrote:
> I intend to raise polyclonal antibodies in mice against a plant fungal
> pathogen. I can't use Rabbits because the enzyme kits I will be using are
> designed to be used with mice.
>> Can someone suggest a suitable protocol for immunizing the mice.
>> How much antibody can I expect to collect from the blood serum
> of one mouse?
>> Is it enough to conduct an assay for polyclonal antibody activity?
>> Any replies much appreciated.
>> Thankyou.
>> Vidyani
> State Agricultural Biotechnolgy Centre
> Murdoch University,
> Western Australia
>>
