works fine we have done it. I confess we gave up when we got a
phophoimager it's a lot better
jeff frelinger
On Mon, 21 Aug 1995, Rich Miller wrote:
> I need some advice on instrumentation for quantitation of autoradiograms.
> We've been using an 8-year old Molecular Dynamics laser densitometer, and
> it's now broken and in need of expensive repair. We're thinking of
> replacing it with a new densitometer. We mostly do autoradiograms of 1-D
> electrophoresis.
>> 1. It seems to me that the easiest (and cheapest) alternative is to buy
> a grey-scale scanner that can create computer-readable TIF or BMP files,
> and then use an image analysis program (like SigmaGel?) to convert the
> density values to area-under-curve or volume measures? But I don't know
> enough about the physics or the scanning system to know if I'll get
> accurate quantitation with the approach: will the numbers in the TIF or
> BMP files be well calibrated with the density in the original image?
>> 2. Do you have any specific recommendations about advantages or
> disadvantages for specific scanners?
>> 3. Do you have any recommendations about dedicated densitometry systems?
>> Any advice appreciated. You can reply to millerr at umich.edu.> Thanks.
>>>>
