sl16s at cc.usu.edu wrote:
>> I want to use RNA from the IL-2 producing cell line( mouse) to use as
> a control along with RNA from mouse splenocyte RNA in my Northern Blots. To
> my knowledge I found EL-4.IL-2 (mouse thymoma) and LBRM-33.4A2 (mouse lymphoma)
> cell lines, which produce good amounts of IL-2. I would like to Know, 1. which
> cell line is best for production of mouse IL-2 mRNA?. 2. Which is easier to
> grow? 3. Is there anyother cell line which is better than these cell
> lines? and 4. which supernatant is better to grow CTLL-2 (IL-2 dependent) cell
> line?. I wil appreciate your help.
> Sincierly
> Ravi Dugyala.
>Ravi:
I have no experience with mRNA preps from EL-4.IL2, nor do I have
experience with the other cell line you mentioned. But, I do know
that EL4.IL2 is a very good line for producing sups for growth of
CTLL-2 cells. We grow cloned CTL lines in our lab, and have used
EL4.IL2 cells for many years with good success.
FWIW,
Craig
