In article <1995Jan17.132209.834 at chmeds.ac.nz>, rsorg at chmeds.ac.nz wrote:
> Dear netters,
>> I am looking for a non-radioactive system to replace [3]T uptake in
> proliferation assays. Sofar, I tried MTT and Alamar Blue (PHA+IL-2 stimulated
> PBMC) but I was not happy with the results. For Alamar Blue, the signal/back-
> ground ratio was pretty bad, for MTT, I had the same problem and in addition
> a wide variation of the ODs. Are there better systems? Any comment would be
> appreciated.
>> Ruediger
>I think there is a kit from Boehringer that is based on BrdU
incorporation. This is detected with a mAb specific for BrdU
and probably works like an ELISA.
jörg
