In article <3ffqj5$ga1 at newsbf02.news.aol.com> you write:
>What is the potential for utilization of sialylated lewis x as a blocker
>for L-selectin. I know it is the ligand for E & P selectin but does
>anyone know if it can be used for L-selectin ...
Yes, it can be a ligand for L-selectin.
> purification
Don't know. Wouldn't think anyone would have tried this, as making the
neoglycogonjugates is much more difficult and expensive then MAb
affinity chromatography!
> blocking
Well, yes to a certain extent. Depends somewhat on the nature of your
experiment, and the way the your carbohydrate is presented.
> in-vitro function!!
This follows from above. sLex is not, however, the best ligand, nor
blocker of L-selectin. The natural ligand(s) is(are) probably (well,
ok, almost definately) more complex as well.
Later,
Aaron
--
"Nothing more is needed to destroy a man, than the conviction that his
life's work is useless." -Antonin Artaud
awarnock at cmgm.stanford.edu (R. Aaron Warnock)
