We are having trouble with double staining for BudR and CD4 on rat cells. If
we hydrolyze with HCl first, the CD4 epitpope is destroyed (we have titrated
hydrolysis down to the minimum). If we stain first for CD4, the subsequent
hydrolysis destroys the diaminobenzidine precipitate.
If anyone has a reliable protocol, or can ppoint me in the right
direction, I would be most grateful.
Gordon MacPherson
macpherson at molbiol.ox.ac.uk
