In Article <3fk9g4$fku at saba.info.ucla.edu>,
jnakamot at pediatrics.medsch.ucla.edu (Jon Nakamoto) wrote:
>Thanks for the RACE reference, but you'll note that the procedure I
>described is a significant modification of the original procedure
>(reduced background due to use of an amine-modified adaptor which is
>used both for 5' and 3' RACE, and combination of the 5' and 3' RACE
>reactions to get the entire cDNA, using LD PCR).
>>I've spent months trying to get 5' ends of some tough cDNAs, so if
>this procedure works I'll use it. But I refuse to overpay for a kit.
>I'll figure out the nitty gritty myself, but a literature reference
>would sure help.
>>Jon Nakamoto
>Clinical Instructor of Pediatrics/Endocrinology, UCLA
>jnakamot at pediatrics.medsch.ucla.edu
I have tended to avoid kits myself too. I especially feel that they're a bad
idea for grad students who are trying to learn the procedures and gain an
understanding of what's going on. On the other hand, I'll bet ANY kit would
cost much less than your monthly salary. If buying a kit can save you months
of unproductive work, it's well worth the price. Sometimes it's more cost
effective to swallow your pride and buy the kit.
Tracy
