rsorg at chmeds.ac.nz writes:
I've tried MTT, XTT, Orange G and Coomassie based methods but the
best is the BrDU incorporation assay by Boehringer. It is the
most sensitive and as it measures DNA synthesis instead of
protein or mitochondrial activity it is best suited to detect
changes in the cell cycle. good Luck!
>Dear netters,
>I am looking for a non-radioactive system to replace [3]T uptake in
>proliferation assays. Sofar, I tried MTT and Alamar Blue (PHA+IL-2 stimulated
>PBMC) but I was not happy with the results. For Alamar Blue, the signal/back-
>ground ratio was pretty bad, for MTT, I had the same problem and in addition
>a wide variation of the ODs. Are there better systems? Any comment would be
>appreciated.
>Ruediger
>>Ruediger V. Sorg, Ph.D. |
>Christchurch School of Medicine |
>Haematology/Immunology Research |
>Christchurch, New Zealand |
>E-MAIL RSORG at CHMEDS.AC.NZ |
>PHONE #64 (3) 364-0553 |
>FAX #64 (3) 364-0750 |
