RUIZ VAZQUEZ, Elena (ervazquez at mail1.cti.unav.es) wrote:
:_______________________________________________________________________________
: Dina wrote:
: <<Agnieszka Szczepek (aszczepe at GPU.SRV.UALBERTA.CA) wrote:
: : >
: : > Which is the reason why if I add to a PBL culture (without any stimuli,
: only
: : > PBL and RPMIc) mAb anti-DR (L-243) the spontaneous IL-2 releasing goes
: from
: : > 500 pg/ml (more or less) to 50 pg/ml?
: : Would that be anergy??? Looks like it.
: <<No, I think you've rediscovered the syngeneic MLR. The observation was
: <<made a while ago that mouse LN or spleen cells sometimes spontaneously
: <<proliferate in culture. Inhibition of IL-2 release by anti-self class II
: <<Ab would suggest that the syngeneic MLR involves recognition of self MHC
: by T cells.
: <<I assume there are no foreign proteins (such as FCS) in your culture
: medium...
: Yes, there are, 10% FCS.
: Elena
:ervazquez at mail1.cti.unav.es
Then of course, it's possible that there is a primary response to FCS
derived peptides in the context of class II molecules. This should be
very small, but it is a theoretical possibility.
Avinash Bhandoola.
