_______________________________________________________________________________
Dina wrote:
<<Agnieszka Szczepek (aszczepe at GPU.SRV.UALBERTA.CA) wrote:
: >
: > Which is the reason why if I add to a PBL culture (without any stimuli,
only
: > PBL and RPMIc) mAb anti-DR (L-243) the spontaneous IL-2 releasing goes
from
: > 500 pg/ml (more or less) to 50 pg/ml?
: Would that be anergy??? Looks like it.
<<No, I think you've rediscovered the syngeneic MLR. The observation was
<<made a while ago that mouse LN or spleen cells sometimes spontaneously
<<proliferate in culture. Inhibition of IL-2 release by anti-self class II
<<Ab would suggest that the syngeneic MLR involves recognition of self MHC
by T cells.
<<I assume there are no foreign proteins (such as FCS) in your culture
medium...
Yes, there are, 10% FCS.
Elena
ervazquez at mail1.cti.unav.es
