Cell culture is performed generally in a humidified, CO2-incubator. In
order to prepare metaphase spreads, lymphoyctes are cultured for only
three days in the presence of a mitogen and colicimide. I wonder if this
condition requires CO2 or not. Could anybody have experience in culturing
human peripheral lymphocytes for genetic research?
--In C. Kim <inkim at carina.unm.edu>
