I am interested in performing flow analysis on murine splenocytes for
T-cell antigens, B-cell antigens and enteroviruses. We have two
antibodies, a mouse anti-virus monoclonal antibody and a rabbit
anti-virus polyclonal. The CD antigens have worked extremely well
under many conditions, however, we have had problems with
non-specific staining with the anti-virus antibodies.
The mouse MAb is excellent for staining virus, however, in murine
tissue the secondary gives the background. This occurs both with
the conventional direct and indirect staining with respect to a labelled
2' anti-mouse antibody. The non-specificity of the rabbit antibody
appears to be primarily in the rabbit antibody itself as there is no
2' Ab background staining.
If anyone has any comments or suggestions, I
would enjoy discussing these issues.
Dan Anderson (danderson at prl.pulmonary.ubc.ca)
The University of British Columbia
The University of Nebraska Medical Center
