re: staining backgound problems from
>Dan Anderson (danderson at prl.pulmonary.ubc.ca)
>The University of British Columbia
>The University of Nebraska Medical Center
To better understand this problem, it would be necessary to know what
kind of background (what specific types of tissue microenvironments show
background). A prblem with Fc mediated background as suggested by the
last posting has a characteristic appearance due to the distribution of
cells with high FcR levels.
When you say the "CD antigens" have worked well, exactly what do you
mean? Do you use the same secondary reagent for these as for the murine
mAb for the virus?
Pat Bucy (bucy at wp.path.uab.edu)
Dept. of Pathology
Univ. of Alabama at Birmingham
