I have a CHO cell line that produces a chimeric IgG4 that unfortunately has
to be cultured in serum containing media (8% feotal calf serum). This FCS also
contains bovine IgG which I would like to selectively remove from the culture
supernatant. Has anyone had any experience with this problem? I have read that
Pharmacia's Protein G Fast Flow 4 will remove the bovine IgG has anyone tried
it? Finally how about purifying the FCS before addition to the media, is this
feasible or would maintaining sterility of the serum be to difficult (the FCS
has components that would be denatured if autoclaved). Thanks in advance.
Matthew.
--
