I used to do proliferation assay on Immune deficient patients and like
yourself did not want to use the radioactive label. I used flow
cytometry using antibodies measuring proliferation nuclear antigens, the
antibodies used were KI-67 and PCNA. The assay involved adding PHA, ConA,
and PPD to lymphocytes in a 96 well format and incubating for 72 hours
-over the weekend. I have not the method with me as I am sending this
from home. I am sure Sigma and others do such a assay commercially. If
you are interested I could dig deeper.
Ed Yousaf
Bone Marrow Transplantation and Tissue Culture,
Bristol
