In article <frodes-0606951126500001 at tirpitz.ibg.uit.no>, frodes at ibg.uit.no
(Frode Skarstein) wrote:
> This is where you immunology people come in. I would love to hear how you
> guys define "genetic resistance". What heritable aspects of the immune
> defence can enable some host genotypes to deal with a given pathogen while
> other host genotypes succumb?
>> Most aspects of the immune system probably is probably under some degree
> of genetic influence, and could thus be lumped onto the pile "genetic
> resistance". However, I am interested in the aspects of the immune
> function that could potentially create cyclic co-evolution between host
> and pathogen, and for which variation between individuals exists.
Dear
> Frode Skarstein,
maybe this does not help you very much, but lets have it a try:
TI: Natural resistance to infection with intracellular parasites:
isolation of a candidate for Bcg.
AU: Vidal-SM; Malo-D; Vogan-K; Skamene-E; Gros-P
AD: Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
SO: Cell. 1993 May 7; 73(3): 469-85
ISSN: 0092-8674
PY: 1993
LA: ENGLISH
CP: UNITED-STATES
AB: Natural resistance to infection with intracellular parasites is
controlled by a dominant gene on mouse chromosome 1, called Bcg, Lsh, or
Ity. Bcg affects the capacity of macrophages to destroy ingested
intracellular parasites early during infection. We have assembled a 400 kb
bacteriophage and cosmid contig within the genomic interval containing
Bcg. A search for transcription units by exon amplification identified six
novel genes in this contig. RNA expression studies showed that one of
them, designated Nramp, was expressed exclusively in macrophage
populations from reticuloendothelial organs and in the macrophage line
J774A. Nramp encodes an integral membrane protein that has structural
homology with known prokaryotic and eukaryotic transport systems,
suggesting a macrophage-specific membrane transport function.
Susceptibility to infection (Bcgs) in 13 Bcgr and Bcgs strains tested is
associated with a nonconservative Gly-105 to Asp-105 substitution within
predicted transmembrane domain 2 of Nramp.
MESH: Amino-Acid-Sequence; Base-Sequence; Carrier-Proteins-chemistry;
Carrier-Proteins-immunology; Chromosome-Mapping; DNA-genetics; Exons-;
Gene-Library; Genes,-Dominant; Macrophages-immunology;
Membrane-Proteins-chemistry; Membrane-Proteins-immunology; Mice-;
Mice,-Inbred-CBA; Mice,-Inbred-C57BL; Mice,-Inbred-Strains;
Models,-Structural; Molecular-Sequence-Data; Oligodeoxyribonucleotides-;
Organ-Specificity; Polymerase-Chain-Reaction-methods;
Protein-Structure,-Secondary; Restriction-Mapping;
Sequence-Homology,-Amino-Acid; Transcription,-Genetic;
Tumor-Cells,-Cultured
MESH: *Carrier-Proteins-genetics; *Immunity,-Natural-genetics;
*Macrophages-physiology; *Membrane-Proteins-genetics;
*Parasitic-Diseases-immunology
TG: Animal; Comparative-Study; Support,-Non-U.S.-Gov't
GS: Bcg; Lsh; Ity; Nramp
PT: JOURNAL-ARTICLE
RN: 0; 0; 0; 0; 9007-49-2
NM: Carrier-Proteins; Membrane-Proteins; Nramp-protein;
Oligodeoxyribonucleotides; DNA
AN: 93258812
UD: 9308
SI: GENBANK/L13732; GENBANK/S63764; GENBANK/S63803; GENBANK/L09220;
GENBANK/L09221; GENBANK/L09222; GENBANK/L09223; GENBANK/L09224;
GENBANK/L09225; GENBANK/L33709
jorg
kirberg at bii.ch
