In article <m0sHC8e-000DsUC at uctmail.uct.ac.za>, SRESS at uctgsh1.uct.ac.za says...
>>Has anyone tried this assay for measuring IL-4? It is meant to be
>more economical, allowing about 15 plates to be measured, but both we and
>another local lab cannot get OD's above 0,3-depite following the
>recommended protocol.
>>Has anyone got it to work? Any special advice? Any help would be
>gratefully appreciated.
>>Stan
>>Is this to detect human cytokine? We thought about useing these pairs here in Glasgow but
Genzyme use different antibodies in the duoset than in their kits (which always work, but cost
300-400 pounds per plate). This looks abit suspect. We also heard rumours of 'problems with
specificity'.
Now I use a pair of antibodies from Pharmingin. The antibodies work out at about
500-600pounds/pair. You get 500ug of each, which is fairly cheap on a plate by plate front.
They rather unhelpfully don't have any distributers in Africa, but heres the UK contact:
Fax:0223 60732 phone, 0223 316255
Janet Spicer
University of Glasgow
gbzv71 at udcf.gla.ac.uk
