A few years ago I had trouble getting genzyme IL4 elisa to work. It
turns out that there was something wrong with their elisa kit. I
mentioned my problem (I had switched to another kit by that time) to them
in a conference and the reply was "oh yes, there was a problem with that."
I am not convinced that their quality control is adequate. Don't blame
yourselves yet- talk to them first and make sure their kit has been
working. I know they are well known/respected but have refrained from
ordering from them since. The Pharmingen IL4 antibody pairs are good if
you don't mind putting together the rest of the reagents (they have a
protocol- and of course, it is less expensive).
