On Mon, 29 May 1995 kault at tyrell.net wrote:
> Is anyone familiar with a commercially available product that can be used for lymphocyte
> proliferation testing? I am looking for something that is based on a 96 well plate. I also
> would like to avoid using radioactivity. I have tried one product, but the backgroud absorbance
> was very high so it was difficult to get a meaningful proliferation index. Thanks -
> Kevin Ault, M.D. University of Kansas Medical Center (kault at tyrell.net)
>>>
It is possible to use a tetrazolium salt like MTT to evaluate
proliferation cellular responses. The MTT has a Yellow color and is reduce
to a blue formazan in the mitocodria of limphocytes.This assay is
describe in Mosmann' paper(1983) and can be avaluate
specphotometricly(O.D. 570nm).
The reference is: Mosmann, T. R. Rapid colorimetric assay for cellular
growth and survival: Application to proliferation and citotoxicity assay.
J. Immunol. Methods, v.65, p.2348, 1983.
The MTT(3-[4,5 dimethylthuazol-2yl] 2,5-diphenyl) tetrazolium bromide)
and can be purchased from sigma. You can use 10 ul to 100ul of
final volume from a stock solution of 5 mg/ml in PBS.
Cheers
Cosme Malaquias
