We are using the Clontech Rat TNF-alpha primers to amplify TNF in rat.
The GCG sequence indicates that the primers supplied by Clontech should
amplify a fragment ~570 bp while Clontech says the product should be 295bp.
The actual fragment size is 295bp but, why is there a discrepancy? It is
difficult to get any quick answers from Clontech on this issue. Takes a
couple of days to hear back from tech services. Thanks for any input. Mary
