In article <Pine.A32.3.91.950616093107.45612A-100000 at umabnet.ab.umd.edu> "Mary P. Remington" <mremingt at umabnet.ab.umd.edu> writes:
>From: "Mary P. Remington" <mremingt at umabnet.ab.umd.edu>
>Subject: TNFa-anyone sequenced frag. amplified by primers for rat??
>Date: Fri, 16 Jun 1995 09:38:37 -0400
>We are using the Clontech Rat TNF-alpha primers to amplify TNF in rat.
>The GCG sequence indicates that the primers supplied by Clontech should
>amplify a fragment ~570 bp while Clontech says the product should be 295bp.
>The actual fragment size is 295bp but, why is there a discrepancy? It is
>difficult to get any quick answers from Clontech on this issue. Takes a
>couple of days to hear back from tech services. Thanks for any input. Mary
GenBank has a rat TNF-alpha entry (Locus=RATTNF) for a genomic sequence that
will give 295 bp once all three introns are removed. Leaving one of the
introns in would give a fragment of ~580.
GenBank also has a cDNA sequence entry (S40199) that would give a fragment
size of 295 except that the sequence corresponding to the 5'-primer differs by
one base. I don't know if the discrepancy is due to a strain difference,
sequencing error, or the fact that the cDNA clone was obtained by PCR.
