Concanavalin A will activate CD8+ cells in vitro but it is a polyclonal
activation so it will not select out for or induce CTL specific to any
particular antigen. There is a fair amount of literature on Con A
activation of human T cells and its resultant effects. A older (1977)
reference for long term cultivation of CTL's is Gillis and Smith, Nature,
268; 154-156. I don;t know if this helps as you might be interested in
"inducing" antigen-specific CTL in vitro, in which case you'll need to dig
deeper.
Hope this helps some.
Jerry Pier
Harvard Medical School
In article <3rscne$1cf at news.cerf.net>, Esteban Celis
<esteban_celis at cytelcorp.com> wrote:
> Does anyone have suggestions on how to induce CTL in vitro using
> lymphocytes from naive individuals?
