IUBio

IEL isolation and purification

Randall H. Friedline friedr at med.unc.edu
Mon Jun 19 12:01:39 EST 1995


 Hello again!
I plan on isolating IEL's (gamma-delta +) from mice.  I was 
wondering why all the established procedures use the small 
intestine as the source of IEL's but not the large intestine.  I 
need to purify IEL's from the large intestine.  Is there 
something I'll have to be aware of when performing the 
procedure that differs from removal from small intestines??
  Are cell yields lower from large intestine than small 
intestines?
  Thanks...

-- 

     Cheers,
        Randy

   friedr at med.unc.edu               " I am Homer of Borg.
  (.sig stolen from someone)          Resistance is.......
                                      ...Mmmmmm..donuts!!"





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