I am trying to separate microglia cells
from primary astroglia cultures. Following
dissection of cortical cells from 3 day old
mice, they were plated and grown to confluency
for 12 days. I have tried to separate the
microglia cells from the astroglia cultures
by first shaking the flasks at 180rpm for 2hrs and
then filtering the resulting cell suspension
through a 20um filter. The cell population
which results is very sparse and only remains
viable for 2-4 days. Does anyone have
suggestions on how to increase the yield of microglia
cells and also their viability?
