Hybridoma production failure

[dr. jack komisar]

            I read somewhere that myeloma parent cells can revert to
          HAT resistance, and that it is a good idea to re-select
          HGPRT negative cells periodically for their resistance to
          20 fg/ml of 8-azaguanine and to re-select thymidine
          kinase negative cells for their resistance to 30 fg/ml of
          BrdU.  However, aminopterin resistance may not be a
          common problem.  Reversion of the X63-Ag8 line is rare, if
          it occurs at all.


                                                     Jack Komisar