At 10:26 PM 95.3.5 +0000, Jeffrey Upperman wrote:
>does anybody know if rat tumor necrosis alpha cross reacts with human
>endothelial cells or polymorphonuclear cells?
>>thanks
>--
>Jeffrey S. Upperman,MD Internet: upperman at umdnj.edu>Research Fellow Voice: (201) 982-4474
>Dept. Anatomy, Cell Biology & Injury Fax: (201) 982-6803
>Dept. of Surgery Beeper: (201) 708-4150
>New Jersey Medical School Office: (201) 982-5045
>185 S. Orange Ave., G606 Lab: (201) 982-5404
>Newark, NJ 07103
Dear Sir;
I think it does. But you need attention and careful examination to
prove it. Evidences ever elucidated are:
1. Human TNF (TNF-alpha) binds to and triggers signals of 55-kDa but not
75-kDa mouse TNF-R. Therefore, human TNF is weaker than mouse TNF in some
activities of mouse system.
2. Mouse TNF binds to and triggers signals of at least 55-kDa human TNF-R.
According to abve these data, rat TNF is expected to bind and signal at
least 55-kDa human TNF-R, which works as the main signaling TNF-R as for
endothelial cells and PMN. But, I am not sure if mouse TNF binds to 75-kDa
TNF-R or not. In addition, I don't think you can find out data on rat TNF
in recent articles. Though it depend on your experiment system, you might
need not to clear these points. Anyway, I think it does. Try.
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