Try using buffer instead if water in your agarose. For a detailed
discussion see Chp 32 ofthe handbook of experimental immunology (Weir ed)
blackwell 1986.
Jeff Frelinger
On Fri, 3 Mar 1995, Lewis X. Liu wrote:
> I needed suggestions on how to make precipitin zones visible to the naked eyes
> in agar gels. I know that antibodies reacted to its specific antigens forming
> precipitin zones in agar gels (3% agar in water), but I have trouble making it
> visible to the naked eye. Is there any reagents that I could use to make
> these precipitin zones visible (such as dyes, etc.)? Any suggestions are
> appreciated. Thank you.
>>>> =================================================================
> _ _/| | Love is the triumph of imagination over
> \'O O| | intelligence.
> ={_:_}= meow! | - H. L. Mencken
> ==============|==================================================
> Lewis X. Liu | xliu01 at barney.poly.edu> =================================================================
>>>
