I seem to have a problem with PIPLC use in culture.
According to a paper on the purification and properties of the PIPLC from 'Bacillus
Thuringiensis', KCL and NaCL at >[10mM] are inhibitory for PIPLC.(Biochimica&Biophysica Acta,
619,1980,48-57)
I want to use it on some brain endothelial cells in culture (96 well format) to see if the
antigen I am interested in is PIPLC sensitive or not (by cell surface ELISA). Most buffers(HBSS,PBS)
and/or media (HAMs F10, MEM) I can think of using to keep my cells going during the digestion have
between 100-150mM NaCL!
Any ideas? Am I just reading an outdated paper?
I would very much appreciate any help.
Sincerely,
Galatia Politopoulou.
PS SIGMA technical services who sold me the stuff couldn't help! Ah well!
