shopkins at fs1.ho.man.ac.uk (Steve Hopkins) wrote:
>> We have some IL-4 knockout mice that have had their gene inactivated by
> insertion of a bacterial neomycin resistance gene. This is a fairly
> standard procedure and I wonder if anyone could direct me to information
> about the sequence of the gene that is used. We are trying to understand
> the reason for an odd PCR product we are getting from the DNA of these
> mice.
>> Thanks
>> Steve Hopkins
>
Steve:
Although I don't know exactly which cassette was used, more than
likely, a pMC1Neo neo fragment was used in the production of these
knockout mice. You can get the sequence for the neo resistance
gene from the GenBank databases; look under neomycin
phosphotransferase. Another option is to call up Stratagene. They
have the entire sequence of the gene on their computers and they
can provide you with a copy. If you need more info, let me
know.
Craig Walsh
UCLA
