I have some ideas on the detection and purification of newly expressed protein.
Instead of using cDNA subtraction or 2D PAGE analysis, I will use immunosubt
ractive methods. The princeple of this method is producing the polyclonal
antibodies against whole protein mixture of tissue or cultured cell and select
ing the antibodies against newly expressed protein by passing the antiserum
against the protein(antigen) column of control tissue of cell line. After immuno
subtraction, the newly expressed protein will be easily purified by immuno
precipitation and detected on SDS PAGE gels. Dose anybody advise me about this
idea? Any Comments will be greatly appreciated. Thanks for reading.
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KAIST, Deajon Korea. Dooyeon Kim ......................
dykim at bioneer.kaist.ac.kr
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