ingen at mack.rt66.com wrote:
: Hi all,
: I have a simple question. Which dyes are better for counting nucleated
: cells (e.g., live + dead lymphocytes)? Trypan blue is only used to count
: dead cells. Any comments are welcomed.
If you have a fluorescence microscope, ethidium bromide and acridine orange
will distinguish live cells from dead ones. The live ones will stain green
while the dead ones stain orange under fluorescence.
TKendrick
