In article <3p8bom$goa at cisunix1.dfci.harvard.edu>, "Matthew P. Frosch, MD, PhD" <frosch at dsg.harvard.edu> writes:
>I am soliciting advice about what companies provide good service for
>production of antisera to recombinant proteins. I have used one
>company (they were cheap) for three different fusion proteins and
>not a single sera (for 5 animals) was useful in Western blotting or
>immunofluorescence.
>>What services have people liked?
>>What form did you submit your material in?
>>What animal did you use (the proteins are all human in origin)?
>>Thanks in advance (as always).
>> Matthew
>>I have used Pocono Rabbit Farms for production of bunny antisera to purified
native and recombinant proteins, and they usually do quite well.
717-595-7108
Good luck!
________________________________
Benjamin A. Kruskal, MD, PhD
Division of Infectious Diseases
The Children's Hospital
300 Longwood Ave.
Boston, MA 02115
617-355-5152
617-355-6575 (fax)
kruskal at a1.tch.harvard
________________________________