In article <D9D10s.B6n at tyrell.net> , kault at tyrell.net writes:
>Is anyone familiar with a commercially available product that can be
used for lymphocyte
>proliferation testing? I am looking for something that is based on a 96
well
>plate. I also
>would like to avoid using radioactivity. I have tried one product, but
the
>backgroud absorbance
>was very high so it was difficult to get a meaningful proliferation
index. Thanks -
>Kevin Ault, M.D. University of Kansas Medical Center
(kault at tyrell.net)
By far the most widely used, cheapest and most reliable lymphocyte
proliferation based in 96 well plate is tritiated thymidine. Harvesting
is via glass filter paper using one of several automatic harvester
systems. We have a Packard which harvests whole plate at one time and
then reads the paper as is. No need for scintillation fluid etc....
