In article <3qhnbr$j2d at netnews.upenn.edu>, David Peritt
<Peritt_d at a1.mscf.upenn.edu> wrote:
> In article <D9D10s.B6n at tyrell.net> , kault at tyrell.net writes:
> >Is anyone familiar with a commercially available product that can be
> used for lymphocyte
> >proliferation testing? I am looking for something that is based on a 96
> well
> >plate. I also
> >would like to avoid using radioactivity. I have tried one product, but
> the
> >backgroud absorbance
> >was very high so it was difficult to get a meaningful proliferation
> index. Thanks -
> >Kevin Ault, M.D. University of Kansas Medical Center
> (kault at tyrell.net)
I tried Promega's nonradioactive kit, and the readings I got were low (all
around) and fairly inconclusive. I also wanted to avoid radioactivity,I
broke down and did the tritiated thymidine method and got very good
results. I had tried the kit a few times, so it wasn't only a one time
thing. I don't know if the thymidine method is the cheapest, but it does
seem to be very reliable. The only thing is you have to also have a
harvester.
