(Robert Burns) wrote:
> Hi all,
> I wondered if anybody has used a product called ABX produced
> by J and T Baker for Mab purification. I is some kind of silica
> affinity gel which is supposed to bind all isotypes. Anybody tried
> it? I am particularly interested in IgM purification.
> Rob
> Robert Burns
>> Monoclonal Antibody Unit
> Scottish Agricultural Science Agency
> East Craigs
> Edinburgh
> Scotland
>>burns at sasa.gov.uk
Rob,
I have just begun to use this product (Bakerbond ABx Prepscale) to purify
an IgM Mab and it seems to work well although I have not run a gel on the
final product to check for absolute purity. However, it does give a
decent separation from the great majority of FBS proteins by analysis of
protein and immunoreactive peaks. Works for murine IgG and human IgG's
also, but again final purity unknown.
Mike
--
Michael Preston
Channing Laboratory
preston at warren.med.harvard.edu
