>Does anyone know if in B-cell differentition, the D-J recombination and
>the Variable Heavy-DJ recombination reactions have been successfully
>performed in-vitro. A friend of mine said that he thought Dr. Stuart
>Kauffman of the Santa Fe Institute has done this, but I haven't been
>able to find any references on this. Any help would be greatly
>appreciated.
No. It has not been done.
M.Sadofsky (spell?) and coauthers published
a couple of months ago in Cell a paper on V(D)J recombination
in vitro but.... it was very far away from total success.