In article <dtyyu.106.00089808 at ucla.edu>, dtyyu at ucla.edu (David Tak Yan
Yu) wrote:
>I wish to culture hybridoma in serum free media to allow me to isolate the
>antibody. Can some one recommend a U.S. manufacturer?
>David Yu
We use HB101 serum-free (not protein-free) medium from Irvine Scientific
for making our antibodies. You may need to passage your cells in mixtures
of serum-containing/serum-free medium for several passages to "wean" them
off serum. However, many lines just grow fine directly in HB101. Good
luck!
____________________________________________________________________________
Richard R. Hardy Member, Institute for Cancer Research
Fox Chase Cancer Center Tel: (215) 728-2463
7701 Burholme Ave. FAX: (215) 728-2412
Philadelphia, PA 19111 E-MAIL: R_HARDY at fccc.edu
