IUBio

serum free media for hybridoma

Paul D. Boyer, Ph.D. boyer at cs.uwp.edu
Mon Nov 13 14:04:21 EST 1995


I can recommend against one product--Cellgro Complete sold by Fisher. 
 I used this in a class to demonstrate the need to wean a hybridoma 
into serum-free medium, and the cells refused to multiply in this 
medium.  Regardless of the weaning protocol, the cells remained viable 
but never grew.  As far as antibody production, I can't say as I 
haven't tested that.
-- 


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Paul D. Boyer, Ph.D.                University of Wisconsin-Parkside
Assistant Professor                          900 Wood Road, Box 2000
Department of Biological Sciences            Kenosha, WI  53141-2000
School of Science & Technology                      boyer at cs.uwp.edu
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