Hello-
Firstly, I'd like to thank every one who gave me in put
on my last posting(a question about BrdU staining for Flow),
I recieved several very helpful and thoughtful responses.
My current problem involves removing T-cells from B-
cell preps. I have been doing primary B-cell culture
experiments on cells isolated from human blood. I isolate
them with Ficoll and AET-SRBC rosetting. This has been
working pretty well with 85% plus purity.
Unfortunately, I have to culture them with various
cytokines for more than a week. The contaminating T-cells
tend to take off like a rocket and quickly out compete the
B-cells and may be influincing the progress of the culture.
My question is does any one have a simple, quick
protocol to remove these last few T-cells? I would prefer
something that does not beat to heavily on my B-cell yeild.
and is suitable for between 5 and 20 million cells.
Thanks in Advance,
Richard Palmer
Palmer at MSVAX.MSSM.EDU
Box 1198
Mt. Sinai Med. Center
One Gustav Levy Place
New York, NY 10029
New York, NY 10029
