Troels Wind wrote:
>>Heres a little theoretical question for you immunologists. Imagine, that
two
>monoclonal Abs towards the SAME antigen are available. For simplicity,
lets
>assume the antigen is monomeric, i.e. no epitopes exist in duplicate.
>If one uses these two Abs in ELISA towards the antigen under the same
>conditions (same coating, same concentration of Ab, same detection Abs
etc.),
>and one of them gives a higher signal than the other, is it then fair to
say
>that it has a higher affinity than the other? If not, then WHAT is higher
or
>better, yhat results in the better ELISA-signals?
>I would really appreciate any input on this!
If your detection antibody (Goat anti-mouse AP conjugate, e.g.) binds
better to one of the two antibodies, that antibody will give you a higher
signal in ELISA, all other things being equal. Just a question of more AP
molecules per molecule of monoclonal.
Hope this helps.
Mark
