After PCR anplification, chloroform extraction and column purification.
The 260/280 readings for my samples are between 2 and 4 (14 samples).
I've asked around about this, only to get two different opinions. 1)
The samples are very pure. 2) The samples are contaminated.
If I chloroform extract and ethanol/isopropanol extract my PCR products,
the 260/280 readings are between 1.7 and 2.
Any suggestions?
