Hi, there,
We are going to try to express a mouse single-chain antibody in E.Coli.
I know there are many primes can be used for RT-PCR and synthese antibody
gene. However, I would appreciate if somebody can tell me which one I should
start with and which expressing plasmid I should use to clone the gene in.
Please reply to this account. Thank very much.
William Jia
